This volume of Methods in Enzymology brings together a comprehensive collection of experimental approaches and mechanistic insights into enzymes work on RNAs—molecular catalysts at the heart of RNA metabolism, regulation, and therapeutics. Enzymes work on RNAs are essential for a wide array of biological processes, from gene expression and RNA processing to translation control, RNA silencing, and genome integrity. The volume is organized into six thematic sections that reflect the multifaceted roles of RNA enzymes in biology and medicine. Together, these chapters—authored by leading experts in the field—provide an essential resource for researchers seeking to understand and manipulate RNA enzyme function using state-of-the-art biochemical, biophysical, and therapeutic approaches.
Section 1: RNA enzymes in transcription, RNA processing, and translation regulation
1. RNA transcriptase in gene expression regulation
Jessie Yan Zhang
2. Enzymes Involved in Ribosome Biogenesis and Translation Regulation
Dmitri N Ermolenko
3. In Vitro Transcriptase: Methods for Studying RNA Synthesis
Lijun Zhou
Section 2: RNA Enzymes in RNA Silencing and Regulation
4. RNA Silencing Machinery: Mechanisms of RISC and MicroRNA Regulation
Alicia Rogers
Section 3: Enzymes in RNA-Based Therapeutics
5. Antisense Oligonucleotides (ASOs) and RNase H: Mechanisms and Applications
Kathy Liu
6. RNA Enzymes in Targeted Therapeutics: From Bench to Bedside
Yuru Wang
Section 4: Enzymes in RNA Sequencing and Analysis
7. Enzymes in High-Throughput RNA Sequencing: Applications and Challenges
Yaming Hou
Section 5: Advanced Techniques and Future Directions
8. Innovative Methods for the Study of RNA Enzymes
Huiqing Zhou
9. Emerging RNA sequencing methods
Jia Sheng
10. RNA Enzymes in Disease Mechanisms and Therapeutics
Mike Kearse
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