Immuno-oncology and immunotherapy, Part D, Volume 196 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Investigating the endothelial-immune axis in cancer, Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter Cell Lines for Cancer Immunology and Therapeutic Applications, Amino acid analog-based labelling of the immunomodulatory cancer secretome, Human and mouse iNKT cell expansion and engineering with viral vectors, Next-generation deconvolution of the tumor microenvironment with omnideconv, and much more.
Additional sections cover Comprehensive prediction of tumor neoantigens with NextNEOpi, Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic culture, A flow cytometry-based assay for evaluation of T cell activation and degranulation in coculture systems, An adenosine production assay suitable for 2D and 3D cancer cell culture, Determination of lactate in the tumor interstitial fluid, Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils, Organ-on-Chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells, and much more.
1. Investigating the endothelial-immune axis in cancer
Gabriele Bergers and Kathryn A Kathryn A. Jacobs
2. Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter Cell Lines for Cancer Immunology and Therapeutic Applications
Raymond Birge
3. Amino acid analog-based labelling of the immunomodulatory cancer secretome
Karin E. de Visser, Annemieke C. Bouwman, Antoinette van Weverwijk, Onno B. Bleijerveld, Liesbeth Hoekman, Bob J. Ignacio and Kimberly M. Bonger
4. Human and mouse iNKT cell expansion and engineering with viral vectors
Gloria Delfanti, Paolo Dellabona, Giulia Casorati and Michela Consonni
5. Next-generation deconvolution of the tumor microenvironment with omnideconv
Francesca Finotello, Markus List, Alexander Dietrich and Lorenzo Merottto
6. Comprehensive prediction of tumor neoantigens with NextNEOpi
Francesca Finotello, Dietmar Rieder and Markus Ausserhofer
7. Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic culture
Russell W. Jenkins
8. A flow cytometry-based assay for evaluation of T cell activation and degranulation in coculture systems
Andreas Lunqdvist
9. An adenosine production assay suitable for 2D and 3D cancer cell culture
Andreas Lunqdvist and Apple Tay Hui Min
10. Determination of lactate in the tumor interstitial fluid
Santos Manes, Ainhoa Ruíz-Iglesias, Angel García-Aldea, Elena Nonnast and Rosa M. Peregil
11. Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils.
Fabrizio Mattei and Giovanna Schiavoni
12. Organ-on-Chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells
Fabrizio Mattei, Giovanna Schiavoni, Francesco Noto, Adele De Ninno, Mario Falchi and Luca Businaro
13. Evaluating transcriptional TME responses to immunotherapy using microarray analysis
Karen Mossman
14. Expansion and characterization of human peripheral blood derived NK cells for functional studies on tumour organoids
Julian Pardo Jimeno Sr. and ARIEL RAMIREZ LABRADA
15. Inflammatory Neovascularization Analysis In Vivo in Matrigel Implants with Isolated IDVCs (IDO1-Dependent Vascularizing Cells)
Alexander J. Muller, Souvik Dey, Arpita Mondal, James DuHadaway and Shih-Chun Shen
16. Phenotypic assessment of dendritic cell maturation by cost-effective custom ELISA assays
Peng LIU, Guido Kroemer, Oliver Kepp and Liwei Zhao
17. Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells
Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang
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