The second edition of the textbook Enzymes- Catalysis, Kinetics, and Mechanisms focuses on the two broad mechanistic facets of enzymology namely, the chemical and the kinetic. It endeavors to bring out the synergy between enzyme structures and mechanisms. Written with a self-study approach in mind, the emphasis is on how to begin experiments with an enzyme and subsequently analyze the data. The book is divided into six major sections– 1) Enzyme Catalysis – A Perspective, 2) Enzyme Kinetic Practice and Measurements, 3) Elucidation of Kinetic Mechanisms, 4) Chemical Mechanisms and Catalysis, 5) Exploiting Enzymes, and 6) An end piece on Frontiers in Enzymology. The individual concepts are treated as stand-alone short sections. In case the reader needs to use any one concept, it should be possible with minimal cross-referencing to the rest of the book. Further, the book presents specialized techniques and complex approaches that require involved experimentation in theory with suitable references to guide the reader. The book is proposed more as a textbook in a self-learning mode to students of modern biology, particularly those with limited exposure to quantitative aspects and organic chemistry.
1. Enzymes -Their place in Biology.- 2. Enzymes - Historical aspects.- 3. Hallmarks of an enzyme catalyst.- 4. Origins of enzyme catalytic power.- 5. Which enzyme uses what tricks?.- 6. Structure and catalysis: Conformational flexibility and protein motion.- 7. On enzyme nomenclature and classification.- 8. Chemical Kinetics: Fundamentals.- 9. Concepts of equilibrium and steady state.- 10. ES Complex and Pre-steady-state Kinetics.- 11. Principles of Enzyme Assays.- 12. Good Kinetic Practices.- 13. Quantification of Catalysis and Measures of Enzyme Purity.- 14. Henri–Michaelis–Menten Equation.- 15. More Complex Rate Expressions.- 16. Enzyme Kinetic Data: Collection and Analysis.- 17. Approaches to Kinetic Mechanism: An Overview.- 18. Analysis of Initial Velocity Patterns.- 19. Enzyme Inhibition Analyses.- 20. Irreversible Inhibitions.- 21. Reversible Inhibitions.- 22. Alternate Substrate (Product) Interactions.- 23. pH Studies with Enzymes.- 24. Isotopes in Enzymology.- 25. Isotope Effects in Enzymology.- 26. From Kinetic Data to Mechanism and Back.- 27. Chemical Reactivity and Molecular Interactions.- 28. Acid–Base Chemistry and Catalysis.- 29. Nucleophilic Catalysis and Covalent Reaction Intermediates.- 30. Phosphoryl Group Chemistry and Importance of ATP.- 31. Enzymatic Oxidation–Reduction Reactions.- 32. Carboxylations and Decarboxylations.- 33. Electrophilic Catalysis and Amino Acid Transformations.- 34. Free Radicals and radical enzymology.- 35. Integrating Kinetic and Chemical Mechanisms: A Synthesis.- 36. Enzyme Inhibitor Design: Drug Discovery.- 37. Exploiting enzymes: Technology and applications.- 38. Regulation of Enzyme Activity.- 39. In Vitro Versus In Vivo: Concepts and Consequences.- 40. Future of Enzymology: An Appraisal.
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