Molecular Approaches for the Detection of Fungal Phytopathogens

Edited by Sreenivasa Marikunte Yanjarappa,Deepa Nagaraju,Charith Raj Adkar Purushothama

ISBN13: 9781032503950

Imprint: CRC Press

Publisher: Taylor & Francis Ltd

Format: Hardback

Published: 23/04/2025

Availability: Not yet available

Description
The book discusses recent advancement and updates in the molecular diagnostics of fungal phytopathogens. The techniques covered in the seventeen chapters helps in understanding the importance of early detection of fungal pathogens and their toxins in both post and pre-harvest stages of crop production. The chapters describe the fungal identification and isolation from different sources such as food, feed and cereals using molecular approaches like variants of Polymerase Chain Reaction (PCR) involving generic (genus and species) and gene specific primers, RNA-sequence-based next generation sequence, microarray, Serial Analysis of Gene Expression, Fluorescence In Situ Hybridization, Loop-mediated isothermal amplification, Macroarray, PCR-Denaturing Gradient Gel Electrophoresis, Magnetic Capture Hybridization-PCR, nanotechnology, immunoassays providing faster and sensitive approach than traditional methods that overcome the challenges by developing novel disease control strategies. Features Explores various diagnostic strategies for phytopathogens. Discusses early detection methods when fungal spores are low or no visible symptoms. Provides a historical perspective and compares the traditional methods of detection with the current molecular techniques. Describes details protocol of every techniques described. Covers point-of-care testing for fungal pathogens and quality assurance for molecular testing in plant health.
1: Fungal phytopathogens and importance of their early detection through conventional methods. 2: Fungal phytopathogens diagnostic: A historical perspective. 3: Methodology for the Isolation and Identification of Fungal Pathogens. 4: Polymerase chain reaction and its variants as detection tool for fungal phytopathogens. 5: Loop-mediated isothermal amplification (LAMP) for the detection and quantification of fungal phytopathogens. 6: Oligonucleotide array approaches for the detection of plant pathogenic fungi. 7: An overview on application and advancements of lateral flow assay technique in effective detection of fungal phytopathogens. 8: DNA Barcoding for Identification of Fungal Pathogens. 9: Next Generation Sequencing, a new opportunity for discovering fungal phytopathogens. 10: In-situ hybridization for the detection of fungal phytopathogens. 11: Serological assays for the detection of phytopathogenic fungi. 12: Detection of fungal phytopathogens by nanotechnology method. 13: Aptamers: A Promising Tool for the Detection of Phytopathogenic Fungal Toxins. 14: On-site diagnostic techniques for fungal pathogens. 15: Policies, Regulations and Quality Assurance Requirements for Sustainable Agriculture. 16: Policies, Regulations and Quality Assurance requirements for the agri-food industry. Chapter 17: Further Directions in Fungal Phytopathogen Diagnostics.
  • Microbiology (non-medical)
  • Mycology, fungi (non-medical)
  • Botany & plant sciences
  • Biotechnology
  • Agriculture & related industries
  • Plant pathology & diseases
  • Professional & Vocational
  • Tertiary Education (US: College)
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List Price: £150.00