This volume covers the latest advancements in the study of lipid signaling. The chapters in this book cover topics such as neuronal cells such as retinal ganglion cells, microglial cells to muscle, and epithelial cells; extraction for signaling studies, including clinical studies in humans; and discussions on different cells, tissues, and diseases like Alzheimer’s disease, musculoskeletal diseases, eye diseases, and metabolic diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Lipid Signaling: Methods and Protocols is a valuable resource for researchers looking to expand their knowledge of this important and developing field.
The Interplay of Lipid Signaling in Musculoskeletal Crosstalk: Implications for Health and Disease.- Zebrafish as a Model for Lipidomics and Similar.- Optimized Lipidomics Extraction of Sphingosine and Sphinganine from Optic Nerve for Signaling Studies.- Analysis of Sphingosine and Sphinganine from the Aqueous Humor for Signaling Studies Using Ultra High Performance Liquid Chromatography-Mass Spectrometry.- Nanoparticles and Their Applications in Lipid Signaling.- Market Needs and Methodologies Associated with Patient Lipidomic Diagnoses and Analyses.- Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling.- Isolation of Mitochondria from Murine Skeletal Muscle.- Effects of a Laparotomy on Targeted Lipidomics Profiles in a Mouse Model of Surgical Stress During Aging.- Post-Translational Prenylation Facilitates Proteasomal Degradation of Monomeric GTPases.- α-Linolenic Acid Vesicles-Mediated Tau Internalization in Microglia.- Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation.- Synthesis of Azo Analogs for Investigating Phosphatidic Acid-Mediated Signaling.- Method to Assess the Intracellular Fate and Bioavailability of Clusterin using Live Cell Confocal Microscopy Imaging.- Detailed Protocol for Solid Phase Extraction for Lipidomic Analysis.- Analysis of Lipid GPCR Molecular Interactions by Proximity Labeling.- Downregulation of ATP8B2 to Assess Plasmalogen Distribution and Far1 Expression in Primary Trabecular Meshwork Cells.- Diacylglycerol Signaling in Retinal Ganglion Cells.- Quantifying ABCA1/apoA-1 Signaling Pathways with AFM Imaging and Lipidomic Analysis.- Oxidized Low Density Lipoprotein: Preparation, Validation, and Use in Cell Models.- Lipidomics Profiling of the Linoleic Acid Metabolites after Whole-Body Vibration in Humans.- Analysis of Lipoprotein Signaling in Iris Melanocytes.
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