Human Stem Cell Manual: A Laboratory Guide, Third Edition is a comprehensive compilation of “methods that work” for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is an extraordinary laboratory guide for both experienced stem cell research and those just beginning to use stem cells in their work. The editors chose a range of subjects that are critical to stem cell research worldwide and provide introductions for each chapter which discuss the importance of the information in the broad context of stem cell biology. Also included are chapters on the legal and ethical issues that affect stem cell research and researchers. Cross references in each chapter guide the reader to other chapters that may be of interest and emphasize the interrelatedness of the information in the book as a whole. New coverage includes disease modelling, drug development, and other cell therapy applications. The manual is written by foremost experts from around the world for anyone doing research in the field of stem cell biology.
Part I: Basic methods: Maintenance of stem cell cultures 1. Human Embryonic Stem Cell Culture 2. Feeder layers and feeder-free culture 3. Mouse Embryonic Fibroblast Feeder Cells 4. Cryopreservation of hESC Part II: Basic methods: Characterization of stem cells 5. Classical Cytogenetics: Karyotyping 6. Spectral Karyotyping and Fluorescent in situ Hybridization 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns 8. FACS/Cell sorting 9. Fluorescence Immunocytochemical Analysis of Stem Cells 10. Characterization of Stem Cells Using RT-PCR 11. Gene Expression Profiling of Stem Cells by Microarray 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 13. Generation of HESC-Derived Teratomas Part III: Differentiation of stem cells 14. “Embryoid Body” Formation and Neuroepithelia Differentiation 15. Motor Neuron and Dopamine Neuron Differentiation 16. Oligodendrocyte Differentiation from hESC 17. Cardiac 18. Hematopoietic Part IV: Genetic manipulation of stem cells 19. Genetic Manipulation of hESC: Lentivirus Vectors 20. Methods developed for mouse ES cells Part V: Advanced methods 21. Derivation of human ES cells 22. Neural Stem Cell Culture 23. Transplantation of stem cells 24. In vitro fertilization 25. Functional characterization-neural cells Part VI: Practical issues in establishing a human ES cell laboratory 26. Setting Up a Facility for hESC Research Part VII: Essays on stem cell biology 27. Intellectual Property: Owning the Stem Cell 28. Ethical Concerns for Stem Cell Research 29. Guidelines for hESC Research Oversight (ESCRO) Committees
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